Enhanced topoisomerase II targeting by annamycin and related 4-demethoxy anthracycline analogues.

Apoptosis induced by anthracycline antibiotics in P388 parent and multidrug-resistant cells.

The effect of the topoisomerase II inhibitor doxorubicin and its non-cross-resistant analogue annamycin on DNA degradation and programmed cell death was examined in murine leukemia P388 cells. P388 parental cells exposed to various concentrations of doxorubicin and annamycin for 24 h displayed dose-dependent DNA cleavage: at 1 microM, both doxorubicin and annamycin were effective in inducing DN...

SYNTHESIS OF 4-DEMETHOXY-II- DEOXY-ANALOGS OF DAUNOMYCIN AND ADRIAMYCIN Sir: In our study of new anthracycline analogues, after modification of the sugar moiety

Relationship between topoisomerase II-DNA cleavable complexes, apoptosis and cytotoxic activity of anthracyclines in human cervix carcinoma cells.

The use of anthracyclines as antitumor drugs dates back to the 1970s, but the mechanism of the cytotoxicity of these compounds has long been a matter of debate. There is increasing evidence indicating that drug-induced cytotoxicity commonly converges on the induction of apoptosis. Many authors point to the fact that double-strand breaks, resulting from stabilization of cleavable complexes, are ...

Topoisomerase II alpha expression and cytotoxicity of anthracyclines in human neoplastic cells.

Topoisomerase II is ATP dependent enzyme that catalyzes DNA strand passage, the pivotal process in replication, transcription, recombination etc. As part of this breakage and religation process the intermediate generated is a cleavable complex between DNA and topoisomerase II. This complex is the target for topoisomerase II inhibitors like epipodophyllotoxins, actinomycin D or anthracyclines. S...

Apoptosis Induced by Anthracycline Antibiotics in P388 Parent and Multidrug-resistant Cells1

The effect of (he topoisomerase II inhibitor doxorubicin and its noncross-resistant analogue annamycin on DNA degradation and pro grammed cell death was examined in murine leukemia P388 cells. P388 parental cells exposed to various concentrations of doxorubicin and an namycin for 24 h displayed dose-dependent DNA cleavage: at l UM,both doxorubicin and annamycin were effective in inducing DNA br...